Dysregulation of lntracellular Calcium Signalling in Lymphocytes after Exhaustive Exercise

Mooren FCh, Lechtermann A, Fromme A, Thorwesten L, Völker K

Abstract in digital collection (conference)

Abstract

The concentration of intracellular free calcium ([ Ca2+h) is an important messenger within the intracellular signalling cascade of the lymphocyte for linking extracellular stimuli, e.g., a mitogen, to cellular response, e.g., proliferation. Therefore, in the present study we investigated the calcium signalling mechanisms in Iymphocytes before and after exhaustive exercise. Healthy volunteers underwent a treadmill exercise test at 80% of their maximal oxygen uptake until exhaustion. Blood samples were taken before, immediately after, l hour after, and 1 day after the test. After isolation the lymphocytes were loaded with the calcium-sensitive fluorescent dye Fura-2 and the emitted fluorescent light was continuously monitored both in a cuvette spectrometer and in a flow cytometer. Compared to results before exercise the Ca2 + response after Stimulation with anti-CD3 or phytohemagglutinin was slightly decreased immediately after exercise, but was about 35% and 80% higher 1 hafter and 1 day after the test, respectively. Treatment with thapsi gargin, an inhibitor of the sarcoplasmic Ca2+-ATPase, revealed no significant difference.Together, our data support the view that exhaustive exercise influences calcium-dependent signalling processes in lymphocytes which might indicate a higher level of activation as reported previously.

Details zur Publikation

Release year: 1999
ISBN: 0943-917X
Language in which the publication is written: English