Dysregulation of lntracellular Calcium Signalling in Lymphocytes after Exhaustive Exercise

Mooren FCh, Lechtermann A, Fromme A, Thorwesten L, Völker K

Abstract in Online-Sammlung (Konferenz) | Peer reviewed

Zusammenfassung

The concentration of intracellular free calcium ([ Ca2+h) is an important messenger within the intracellular signalling cascade of the lymphocyte for linking extracellular stimuli, e.g., a mitogen, to cellular response, e.g., proliferation. Therefore, in the present study we investigated the calcium signalling mechanisms in Iymphocytes before and after exhaustive exercise. Healthy volunteers underwent a treadmill exercise test at 80% of their maximal oxygen uptake until exhaustion. Blood samples were taken before, immediately after, l hour after, and 1 day after the test. After isolation the lymphocytes were loaded with the calcium-sensitive fluorescent dye Fura-2 and the emitted fluorescent light was continuously monitored both in a cuvette spectrometer and in a flow cytometer. Compared to results before exercise the Ca2 + response after Stimulation with anti-CD3 or phytohemagglutinin was slightly decreased immediately after exercise, but was about 35% and 80% higher 1 hafter and 1 day after the test, respectively. Treatment with thapsi gargin, an inhibitor of the sarcoplasmic Ca2+-ATPase, revealed no significant difference.Together, our data support the view that exhaustive exercise influences calcium-dependent signalling processes in lymphocytes which might indicate a higher level of activation as reported previously.

Details zur Publikation

StatusVeröffentlicht
Veröffentlichungsjahr1999
Sprache, in der die Publikation verfasst istEnglisch
Konferenz36th German Congress of Sports Medicine and Prevention, Freiburg i.Br. Germany, undefined

Autor*innen der Universität Münster

Thorwesten, Lothar
Institut für Sportwissenschaft