Recovery periods during HIIT affect increase in aerobic performance and miR-222 and -29c levels

Schmitz B, Rolfes F, Thorwesten L, Klose A, Krüger M, Brand SM

Abstract in digital collection (conference)

Abstract

Introduction: MicroRNAs (miRs) are short non-coding RNAs that regulate gene expressionas part of the epigenetic machinery. Circulating miRs exert diverse effects on cardiovascularfunction, can be assessed by minimally invasive procedures and are responsive to physical activity.The Sports in Vascular Aging (SPORTIVA) study aims to identify functional miRs as physiologicalmarkers to monitor the efficiency of different cardioprotective exercise regimes.›› Methods: In 2017, the study enrolled 53 young healthy participants who were randomizedinto two training groups using exercise capacity determined by an incremental continuous runningtest (maximum performance test). During a 4-week intervention, two workload-matched HIIT groupsperformed two controlled training sessions/weeks of 4 x 30 s or 8 x 15 s all-out runs. Blood was sampledfrom participants‘ earlobes using a 20 microl capillary with subsequent Trizol extraction. Quantificationof cardiac growth-related miR-222 and -29c was performed using TaqMan MicroRNA assays onan ABI7500 fast real-time PCR system with cel-mir-39 as spike in control for normalization. Results: The comparison of 4 x 30 and 8 x 15 HIIT revealed that longer active recovery periodsof 30 s resulted in greater improvement of aerobic performance (p < 0.0132, pre- vs post-exercise). Theeffect was preceded by elevated miR-222 and -29c levels in the 4 x 30 HIIT group at baseline (both p< 0.01, rest vs post-exercise, R = 0.46) while no effect for both miRs was seen in the 8 x 15 group. Inaddition, a significant increase (both p < 0.001, before vs after training) was detected for resting levelsof both miRs in the 4 x 30 HIIT group. Conclusion: We conclude that longer recovery periods during HIIT might cause greatereffects on aerobic performance. Circulating levels of cardiac growth-related miRs such as miR-222 and-29c are sensitive to different training regimes and may predict physiological training adaptations.Circulating miRs with known cardiovascular functions may thus be used for optimization and controlof physical activities in primary and secondary prevention of cardiovascular disease.

Details zur Publikation

Publisher: Braumann K-M, Bloch W, Böning D, Schnell HJ;
Book title: Deutsche Zeitschrift für Sportmedizin, Abstractband
Release year: 2018
Publishing company: Dynamic Media Sales Verlag
Language in which the publication is written: English
Event: Augsburg
Link to the full text: https://www.germanjournalsportsmedicine.com/fileadmin/content/archiv2018/Heft_5/Abstracts_Sport%C3%A4rztekongress_2018-5.pdf