A metal-mediated base pair that discriminates between the canonical pyrimidine nucleobases

Jash B, Scharf P, Sandmann N, Fonseca Guerra C, Megger DA, Müller J

Research article (journal)

Abstract

A nucleoside analogue comprising the ligand 1H-imidazo[4,5-f][1,10]phenanthroline (P) was applied to develop a molecular beacon capable of discriminating the canonical nucleobases cytosine and thymine. The beacon is based on the formation of a stable Ag+-mediated base pair between P and cytosine, whereas the presence of Ag+ strongly destabilizes nucleic acids comprising an artificial base pair between P and thymine. Metal-mediated base pair formation was investigated by temperature-dependent UV spectroscopy and CD spectroscopy and complemented by extensive DFT calculations. The molecular beacon significantly extends the application spectrum of nucleic acids with metal-mediated base pairs. It is of potential use in the detection of single-nucleotide polymorphisms.

Details zur Publikation

Publisher:
Pages: 7
Release year: 2017
Publishing company: Royal Society of Chemistry
Language in which the publication is written: English
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