Self-assembled multilayer surfaces of highly fluorescent spirobifluorene-based dye for label-free protein recognition

Schlüter F, Ravoo BJ, Rizzo F

Forschungsartikel (Zeitschrift)

Zusammenfassung

The preparation of smart surfaces for protein detection is a challenging field of research. With the aim to achieve label-free detection in the solid state{,} we report on the organic surface functionalization for protein recognition without the need of previous chemical modification of the fluorophore. Layer-by-layer deposition of polyelectrolyte poly(vinyl benzyl tetramethylammonium) chloride (p(VBTMA)Cl) and a tetrasulfonate water-soluble low molecular weight fluorophore (1) based on spirobifluorene leads to modified glass and quartz substrates with outstanding photophysical properties in response to bovine serum albumin (BSA). The absorbance{,} photoluminescence as well as the fluorescence lifetimes were recorded for all surfaces. The surface structure and height of the different number of bilayers polymer/fluorophore have been characterized by atomic force microscopy and ellipsometry. The results show linear trends in the absorption{,} fluorescence and height of the multilayer with increasing number of functionalization steps. Upon incubation with BSA the multilayer shows an increase in fluorescence up to 3-fold{,} which is also detectable with the naked eye. In conclusion{,} we report an easy{,} cheap and biocompatible approach for the construction of protein sensors by self-assembly.

Details zur Publikation

Veröffentlichungsjahr: 2019
Sprache, in der die Publikation verfasst ist: Englisch
Link zum Volltext: http://dx.doi.org/10.1039/C9TB00854C