NADPH cofactor regeneration with surface displayed dehydrogenases

Schüürmann J, Lindhorst F, Jose J

Abstract

One solution to circumvent mass transfer problems and possible side reactions is the presentation of enzymes on the cell surface. The Autodisplay technique replaces the passenger domain of anative autotransporter by a peptide or protein of choice, which is then transported to the cell surface. But bringing an enzyme to the cell surface blocks the access to intracellular cofactors, whichare too expensive to be added stoichiometrically. We used the Autodisplay technology to present dehydrogenases on the surface of E. coli cells for the regeneration of the essential cofactorNADPH. Ultimately, we aim at a combination with a whole cell biocatalyst displaying CYP102A1 to generate an efficient system for biotechnological and pharmaceutical applications.