NADPH cofactor regeneration on the cell surface: an important step to pharmaceutical applications of surface displayed P450 enzymes

Schüürmann J, Lindhorst F, Jose J

Abstract

One solution to circumvent mass transfer problems and possible cross reactions with intracellular enzymes in whole cell biocatalysis is the presentation of enzymes on the cell surface. A currentproject focusses on the display of CYP102A1 from Bacillus megaterium for pharmaceutical applications using the Autodisplay technique [4]. However, the project also elucidated a major challengefor the use of the technology. Bringing an enzyme to the cell surface blocks the access to intracellular cofactors, which are too expensive to be added stoichiometrically. In an approach toconserve the advantages of a surface displayed catalyst, we used Autodisplay to present various dehydrogenases on the surface of E.coli cells for the regeneration of the essential cofactor NADPH.