Erkelenz M, Bauer DM, Meyer R, Gatsogiannis C, Raunser S, Sacc{à} B, Niemeyer CM
Forschungsartikel (Zeitschrift) | Peer reviewedA convenient PCR cloning strategy allows one to prepare hundreds of picomoles of circular single-stranded DNA molecules, which are suitable as scaffolds for the assembly of DNA origami structures. The method is based on a combination of site-directed mutagenesis and site- and ligation-independent cloning protocols, with simultaneous insertion of a nicking endonuclease restriction site on a double-stranded plasmid of desired length and sequence.
Gatsogiannis, Christos | Institut für Medizinische Physik und Biophysik Center for Soft Nanoscience (SoN) |