A facile method for preparation of tailored scaffolds for DNA-origami

Erkelenz M, Bauer DM, Meyer R, Gatsogiannis C, Raunser S, Sacc{à} B, Niemeyer CM

Forschungsartikel (Zeitschrift) | Peer reviewed

Zusammenfassung

A convenient PCR cloning strategy allows one to prepare hundreds of picomoles of circular single-stranded DNA molecules, which are suitable as scaffolds for the assembly of DNA origami structures. The method is based on a combination of site-directed mutagenesis and site- and ligation-independent cloning protocols, with simultaneous insertion of a nicking endonuclease restriction site on a double-stranded plasmid of desired length and sequence.

Details zur Publikation

Jahrgang / Bandnr. / Volume10
Ausgabe / Heftnr. / Issue1
Seitenbereich73-77
StatusVeröffentlicht
Veröffentlichungsjahr2014
Sprache, in der die Publikation verfasst istEnglisch
DOI10.1002/smll.201300701
StichwörterDNA/chemistry; Mutagenesis; Site-Directed; Polymerase Chain Reaction

Autor*innen der Universität Münster

Gatsogiannis, Christos
Institut für Medizinische Physik und Biophysik
Center for Soft Nanoscience (SoN)