Uncomplicated urinary tract infections: The efficacy of extracts from corn silk (Zea mays L.) against uropathogenic E. coli

Rafsanjany N., Sendker J., Lechtenberg M., Petereit F., Scharf B., Hensel A.

Forschungsartikel (Zeitschrift)

Zusammenfassung

Summary The dried corn silk (Maydis stigmata) from Zea mays L. is used traditionally for the treatment of uncomplicated urinary tract infections. A recent screening has indicated that hydroalcoholic extracts of the herbal material inhibit the adhesion of uropathogenic E. coli (UPEC) to T24 bladder cells. For verification of these data EtOH-water (1 : 1) extracts from 4 different batches of Maydis stigmata were investigated. Within an in vitro adhesion assay (UPEC strain 2980 and human T24 bladder cells) a dose-dependent antiadhesive activity against UPEC was verified (IC50 1040 μg/mL). Bioassay guided fractionation of Maydis stigmata by ethanol-water extraction, followed by chromatography on Sephadex LH20 revealed the active fraction XI which was further fractionated by MPLC and preparative HPLC, leading to a still complex subfraction XIG, which was analysed by UHPLC/+ESI-QTOF-MS analysis. Advanced data processing and species-metabolite relationship database revealed the tentative existence of the unusual C-glycosidic flavones derhamnosylmaysin, 3'-deoxyrhamnosylmaysin, 3'-O-methylderhamnosylmaysin, apiferol and alternanthin which might be related to the antiadhesive activity of this subfraction against UPEC.

Details zur Publikation

Herausgeber*innen:
Seiten: 7
Veröffentlichungsjahr: 2016
Verlag: Hippokrates Verlag GmbH
Sprache, in der die Publikation verfasst ist: Deutsch
Link zum Volltext: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84965081932&origin=inward