Traditional herbal drugs against uncomplicated urinary tract infections. In vitro investigations of their anti-adhesive effects against uropathogenic Escherichia coli

Rafsanjany N., Lechtenberg M., Petereit F., Hensel A.

Forschungsartikel (Zeitschrift)

Zusammenfassung

Summary Traditional herbal drugs against uncomplicated urinary tract infections. In vitro investigations of their anti-adhesive effects against uropathogenic Escherichia coli Within a systematic project of medicinal plants, used traditionally against uncomplicated urinary tract infections (UTI), this study investigated anti-adhesive effects of plant extracts against uropathogenic E. coli (UPEC). For systematic evaluation of herbal drugs used against UTI a literature search revealed 275 different plant species. By using a validated selection process 20 species were selected. EtOH-water extracts (1:1) showed no direct cytotoxicity against UPEC (strain 2980) and against the human bladder cell line T24 within the concentration range of 1 to 2000 μg/mL. Five species exerted significant anti-adhesive effects with inhibition of bacterial adhesion to the host cells. Extracts from the rhizomes of Agropyron repens L. and from the stigmata from Zea mays L. reduced bacterial adhesion with IC25 630 μg/mL resp. IC50 1040 μg/mL; the extracts acted against adhesion proteins of the bacteria. Extracts from the leaves of Betula spp. (IC50 415 μg/mL), Orthosiphon spp. (1330 μg/mL) and Urtica spp. (IC25 580 μg/mL) had anti-adhesive effects against UPEC, but interacted with binding proteins of the T24 cells. Combination of extracts with targets on the bacterial side and the host cell had significantly decreased IC50 values (131 μg/mL for combination of Z. mays with Orthosiphon spp. resp. 511 μg/mL for A. repens and Orthosiphon spp.). The data rationalize the use of such phytopharmaceutical preparations, often found in traditional phytotherapy, against UPEC. Combination of these medicinal plants with different targets, as done often in phytotherapy, results in synergistic effects.

Details zur Publikation

Seiten: 7
Veröffentlichungsjahr: 2013
Sprache, in der die Publikation verfasst ist: Deutsch
Link zum Volltext: http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84886572709